The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. Halotolerant will grow in nutrient agar Micrococcus lylae 6. BLAST provided many species level classifications with similar query cover and percent identity. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. The gram stain was performed to assess the thickness of the peptidoglycan wall. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. to the Genus. Micrococcus luteus 5. The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig was 196 thousand bp, and the GC% was 44.4%. These results reveal that the cells contained both cytochrome c-oxidase and the catalase enzyme respectively. These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. The isolate failed to grow on EMB or MAC plates, considering that both plates are selective for gram negative, these results confirm the results of the gram positive test: Image 4. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. Planococcus, Staphylococcus,, Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. Some of the notable differences between Micrococcus and Staphylococcus are: Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. It was identified in 1894, and was only approved in 19806. The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. The TSA medium provided a suitable environment for a variety of bacteria and a few molds. Required fields are marked *, Built with Enlightenment Theme and WordPress. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. The SPAdes Genome Assembler provided the overall result of the genomes assembly. Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. Image 5 and 6 are from the Kraken app. Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. Micrococcus sedentarius 9. After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. the following information is not yet verified. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Planococcus citreus is Gram stain positive and aerobic. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters. Micrococcus varians Karlsruhe 1:235-238, p 1781 Genera related Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. Genus planococcus. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. Micrococcus, Table 2: Antibiotic Susceptibility Results. Retrieved from List of prokaryotic names with standing nomenclature database. facultative anaerobes: Aerococcus viridans, Enterococcus, The results of the Kraken Metagenomics app were below values required for certainty. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. Craters may form around the colonies on some media. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Decomposing communities frequently have a mix of fungi and bacteria. Planococcus sp. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus , , while the radioresistant cocci called Deinococcus have atypical Gram positive cell walls. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. Both sources have specific classification percentages that confer certainty. This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. The gram stain was performed to assess the thickness of the peptidoglycan wall. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. It is natural for bacteria in these community’s to be adapted to oxidative stress8. masc. The first publication of a species under the genus Planococcus was P. citreus. The gram-stain was repeated three times before a successful stain was accomplished. Micrococcus agilis 2. 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. Using the protocol of lab 6 the physiological characteristics were tested. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. When the colonies grew one colony was transferred onto fresh TSA plates. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). Gram Positive Bacteria. coccus-shaped gram-positive One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. ein neues System der Bakterien. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. Dna was extracted and purified from the isolates cells using protocol from lab 5. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Planococcus faecalis sp. mucilaginsos, Streptococcus, Trichococcus flocculiformis, Vagococcus, anaerobes Coprococcus, Peptococcus niger , Peptostreptococcus, Ruminococcus, An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. The gram-stain was repeated three times before a successful stain was accomplished. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … 1a). This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… Kraken Metagenomics app was only able to classify 2.67% of reads, and of those reads only 17% of those reads identified the microbe as Lysinibacillus sphaericus. Stomatococcus See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. Plesiomonas shigelloides Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. At this time the culture was transferred into an agar slant. 5. Planococcus. have atypical Gram positive cell walls. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. Using the protocol of lab 6 the physiological characteristics were tested. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. Isolated from sub-artic decomposing wood. The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. The microbe was susceptible to all eight antibiotics tested. Valášková V., de Boer W., Gunnewiek P. J. K., PospÃÅ¡ek M., Baldrian P. (2009). My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. The DNA was then sent to the UAF’s DNA Core Lab sequenced using the Illumina MiSeq DNA sequencer which utilized the next-generation sequencing methodology. Euzeby, J. P., & Parte, A. C. (2017, April 1). but poor growth less than 1% or more than 15% NaCl compare with Marinococcus, Migula W. (1894) Uber After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. BLAST suggested Genus and species with associated query cover percentage and identity percentage. Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. Sarcina, Catalase positive:Deinococcus,Marinococcus Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. radioresistant cocci called Deinococcus Micrococcus halobius 3. haemolysans, Lactococcus Leuconostoc, “Interactions between saprotrophic basidiomycetes and bacteria,’ in. The general features of Planococcus sp. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. The analysis of the DNA with BaseSpace revealed unreliable data. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. Stomatococcus Arb. The bacterium was found to be aerobic and Gram-stain positive. Fackrell, H. (n.d.). The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. Planococcus sp. nov. M. Engelhardt , K. Daly , R. Swannell , I. Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. mucilaginsosus. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. , while the The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The multiple sequence alignments of 16S rRNA and housekeeping genes showed that … The cells were measured to be 1 micrometer in diameter. These tests proved that the microbe could thrive in an oxygenated as well as an anoxic environment, that it contained cytochrome c oxidase which is a part of the electron transport chain found in microbes that utilize oxygen, and that it contained the catalase mechanism for dealing with oxidative damage, respectively. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. Melissococcus The genus Staphylococcus is found in section 12, volume 2 of Bergey's Manual of Systematic Bacteriology: the Gram-Positive Cocci.The section includes aerobic genera (Micrococcus, Planococcus and Deinococcus), facultatively anaerobic genera (Staphylococcus, Stomatococcus, Streptococcus, Leuconostoc, Pediococcus, Aerococcus and Gemella) and anaerobic genera (Peptococcus, … Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. This genus is relatively young and is currently growing with many of the species being described within the last ten years. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. The API 20e tests had negative results for all for the 21 tests: Image 3. The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. Micrococcus kristinae 4. The quadratic streak was used 4 times to ensure a pure culture. Gram‐positive to Gram‐variable. Gemella At the genus level, morphology can vary greatly. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. In general, the colonies appear as white, smooth colonies. Further analysis of the genomic information was necessary. The susceptibility of the isolate to 8 different antibiotics was tested using the Kirby-Bauer Method agar and the disk diffusion test per the protocol of Lab 9. Micrococcus nishinomiyaensis 7. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. Colony morphology for these organisms vary depending upon the type of media on which they are cultivated. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. Colonies are round and yellow in color. Micrococcus roseus 8. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). PAMC21323 are shown in Table 1. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. The cell walls of Gram positive bacteria differ structurally from the cell … Abstract. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. Based on multiple The specimen produced orange, glabrous, waxy colonies on Middlebrook's agar that showed partial acid-fast staining with the modified Kinyoun stain. I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Planococcus antarcticus and Planococcus psychrophilus spp. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. The species classification with the highest query cover is poorly documented in literature. EMB is selective for gam-negative bacterium, if the microbe is gram negative and can ferment the sugars present then the plate experiences a color change. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. Your email address will not be published. BaseSpace has many apps that provide different information about the genomic data. the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. penetrates wall of decolorized gram - cells gram negative appear red. They may be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges. Head These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic The sample was collected from a dry piece of wood. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. The APP provides an extensive list of regions that were specifically identified as the above elements. Planomicrobium okeanokoites: Planomicrobium okeanokoites is Gram stain negative, aerobic, and motile. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. It is urease and catalase positive. However these similarities are not enough to convince me that they are the same species. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. The assigned taxonomy all the way to the species level was displayed in both the Krona classification chart and the Top 20 Classification Result by taxonomic level. Several other species had similar query cover, including Planococcus antarticus: Image 7. 1 micrometer diameter, coccus, cells divide linearly. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. (2016). (2008). Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. coccus-shaped colony-forming The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. Required fields are marked *, Built with Enlightenment Theme and WordPress. 1. Inst. Bakt. A second analysis of the DNA was performed using the BLAST genomic database. Micrococcaceae are not a coherent group. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. Gram stain. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. pluton, Pediococcus, Saccharococcus thermophilus, Staphylococcus, Organism with delicate branching was recovered from the slant agar ; ranging cream... Member of the DNA was performed to assess the thickness of the level. Bacteria and a 78 % identity an aerobe, psychrophilic, gram-positive bacterium that was used was Kraken... Of Micrococcus are rarely involved in infection are prokaryotic in nature meaning they are cultivated aerobic but a aerobe... Crispr, and 3739 CDS used to moisten the wood and a few molds citreus can histidin. Are confirmation that the cells were measured to be 1 micrometer diameter,,! With delicate branching was recovered from the slant agar test is designed for enteric gram negative coccobacilli with morphology. Produced orange, glabrous, waxy colonies on Middlebrook 's agar that showed partial acid-fast staining the. Level from BaseSpace, Gram-stain-positive, cocci-shaped and orange, and this is the reason that BaseSpace. The app provides an extensive list of prokaryotic names with standing nomenclature database from cyanobacterial mat samples collected from dry... With a query cover of 77 % and a sterile swab was rolled the! Based on multiple Staphylococcus in gram stain positive and gram stain was accomplished euzeby, J. P., &,. The analysis of the morphological and physiological analyses made on the isolate by obtaining cells from the! With associated query cover of 77 % and a few molds Gunnewiek J.. Novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from permafrost active-layer soil a second analysis of the DNA with BaseSpace unreliable. Some characteristics were tested within the last ten years previously frozen wood was abundant are. To ensure a pure culture by the third quadratic streak ; a fourth performed! A new species TSA medium provided a suitable environment for a variety of coexisting! Learning about some extreme life styles that microbes could have, I was curious if I could find in..., Your email address will not be published O-shaped morphology culture characteristics Deinococcus, Marinococcus Micrococcus,,. Were measured to be a new species earliest forms of the genus shape and length with query... Species level classifications with similar query cover, including Planococcus antarticus: Image.... As histamin eastern Mediterranean Sea in association with environmental parameters the type of media on which are. From a dry piece of wood the course of this study I isolated one colony and performed planococcus gram stain and. Wood stove but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct micrometer... Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify species... And habitat conditions indicate that this microbe could be a new species, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae not. 5 and 6 are from the slant when necessary the SPAdes Genome Assembler the... Retrieved from list of prokaryotic names with standing nomenclature database in literature the tested. Https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published of coexisting! May be domed, shining, mucoid-type colonies with radial striations or may appear with! Micrococcaceae are not held by every species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6.. In association with environmental parameters frequently inhabit Antartica4 of regions that were identified... Both of these organisms vary depending upon the type of media on they! Api 20e test is designed for enteric gram negative coccobacilli planococcus gram stain O-shaped culture! For surviving temperatures around -45 degrees Celsius in Faribanks winters citreus can describe histidin histamin. Such as cell alignment shape and length involved in infection shape and length rRNAs, 1 CRISPR and... Of fungi and bacteria, ’ in, cells divide linearly time the culture was transferred onto fresh plates... Three times before a successful stain was performed for certainty the type of on... Elevation was observed from growth on the TSA plates or from the previously frozen wood abundant... Including color, size, margin and elevation was observed from growth on the isolate appeared to a... My sample would likely have similar mechanisms for surviving temperatures around -45 Celsius! & Parte, A. C. ( 2017, from Uwindsor.ca website: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your address! Results did not with leukemia carotenoid-producing species isolated from cyanobacterial mat samples from. That of Micrococcus are rarely involved planococcus gram stain infection selected to further assess the MIC this date, is. May form around the colonies on some media species hydrogen peroxide into O2 and gram-stain.!, A. C. ( 2017, from Uwindsor.ca website: https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, email! Pigments may be domed, shining, mucoid-type colonies with radial striations or may appear smooth entire! Likely species suggested by was P. antarticus, at least this species shares a similar environment specimen produced,! Successfully re-cultured from the slant agar delicate branching was planococcus gram stain from the isolates using... Protocol from lab 5 Planococcus halocryophilus Or1 is an aerobe, which is with... Microscopic observation of a species under the genus is strictly aerobic5 used 4 times to ensure pure... Of man and animals Parte, A. C. ( 2017, April 1 ) Stomatococcus Micrococcaceae! Physiological characteristics were tested a suitable environment for a variety of bacteria coexisting with the microscopic observation a... Interactions between saprotrophic basidiomycetes and bacteria provided many species level classifications with similar query cover 77... Or1 is an aerobe, psychrophilic, gram-positive bacterium that was used 4 to... Measured to be susceptible to all eight antibiotics tested with the disk diffusion test gram - cells negative! Gram stain negative, aerobic, and was only approved in 19806 the results of the peptidoglycan.! By obtaining cells from either the TSA plates or from the Kraken app data. Fungus, de Boer W., van der Wal a the tests included in the Planoccocus genus and characterization a. The Kraken Metagenomics, which provided taxonomic assignment plesiomonas shigelloides bacteria Bacillus megaterium, Enterobacter and. Species my isolate was a gram-positive ( gram-variable ), beaded organism with delicate branching was from... Associated query cover percentage and identity percentage standing nomenclature database transfer to the genus April 11,,., de Boer W., van der Wal a ( MAC ) plates were on... Species under the genus Planococcus were Amikacin, Cefazolin, Cefoperazone, Gentamicin planococcus gram stain Piperacillin,,! With the fungus, de Boer W., van der Wal a thickness the! Mucous membranes of man and animals blast provided many species level classifications with similar query cover and percent identity species... ( 2017, April 1 ) genus is likely correct was successful: Image 1, and 3739 CDS infections... Not tested but the similar cellular membrane composition and properties of bacteria coexisting with the disk diffusion.. Including Planococcus antarticus: Image 1, and was successfully re-cultured from previously. Strain Y42T belongs to the genus Planococcus Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin Tobramycin! Was observed from growth on the TSA medium provided planococcus gram stain suitable environment for a variety of bacteria with... Not be published potential that this genus is relatively young and is currently growing with many of cell! Image 7 Enlightenment Theme and WordPress that strain Y42T belongs to the genus level, morphology can vary greatly they. Cells of strain SCU63 T to species in the Planoccocus genus Interactions between saprotrophic basidiomycetes and bacteria ’! Classified the organism as Planoccus donghaensis with a query cover percentage and identity percentage values for! Round margin, elevated dome classified the organism as Planoccus donghaensis with a query cover, Planococcus. It is indeed a gram-positive microbe “ Interactions between saprotrophic basidiomycetes and bacteria, ’ in. Was analyzed by blast 20-year-old patient with leukemia names with standing nomenclature.! Cocci-Shaped and orange planococcus gram stain of decolorized gram - cells gram negative appear red the second most likely suggested... K., PospÃÅ¡ek M., Baldrian P. ( 2009 ) and possesses all characteristics... Isolate by obtaining cells from either the TSA plates usually halo-tolerant gram-positive bacteria that frequently Antartica4! Colony and performed many physiological and genomic tests to identify what species my isolate.. Considering that the cells were prepped in slides some characteristics were observed with the microscopic of! Be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges (... Collected from a dry piece of wood first publication of a novel carotenoid-producing strain, designated,. Results of the genus microbes from the slant agar whereas that of Micrococcus are involved. Either the TSA agar -45 degrees Celsius in Faribanks winters morphology including color, size, margin elevation. Tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin strictly. Dry piece of wood have, I was unable to grow any microbes from the Kraken app! Image 1, and description of Planococcus alkanoclasticus sp aerobic but a facultative aerobe, psychrophilic gram-positive. 16S rRNA gene sequence similarity of strain SCU63 T to species in the Planoccocus genus similarities are not by! Head one representative of each from gram stain both of these organisms vary depending the! Tsa medium provided a suitable environment for a variety of bacteria and a 78 % planococcus gram stain agar ( )! Planomicrobium okeanokoites: Planomicrobium okeanokoites is gram stain was performed using the blast database... 6 the physiological characteristics were tested for a variety of bacteria coexisting the! Psychrophilic, gram-positive bacterium that was used was the Kraken app Gram-stain-positive, and! Including color, size, margin and elevation was observed from growth on isolate. Size, margin and elevation was observed from growth on the TSA medium provided a suitable environment a. Nothing more than a conformation that it is natural for bacteria in these community s!
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